Guillen was suspended for two games and fined an undisclosed amount for his actions on and off the field following the top of the first inning on Wednesday in a game against the New York Yankees.
Guillen was also disciplined for his comments on Twitter following the ejection which, according to a league press release, "violated Major League Baseball's social media policy and other regulations regarding the use of electronic equipment during the course of a game."
"When I talked to Major League Baseball, I knew exactly where they are coming from, what they want, the integrity of the game, the respect of the game," said Guillen. "It's something they don't want to get out of hand, and I completely understand."
The announcement was made by Joe Garagiola Jr., MLB's senior vice president of standards and on-field operations.
"I think the conversation [with Major League Baseball] was great," said Guillen. "They told me why they were going to do it. Do I regret it? No, not in that moment. The only thing I regret is that I'm going to leave the team by itself for two days.
"I've had a lot of conversations with Major League Baseball over the years. This was the best one I ever had."
Guillen sat out the team's 10-4 loss to Baltimore on Friday and will also miss Saturday's contest against the Orioles. Bench coach Joey Cora is serving as manager during the suspension.
"They will do exactly what they would have been doing as though I was here," said Guillen. "You're going to have a guy after the game that speaks better English than me. That's the only difference."
"I did something I shouldn't be doing, especially during the game. "
Guillen was ejected in the first inning Wednesday at Yankee Stadium. He got into an argument with home-plate umpire Todd Tichenor after Paul Konerko was called out on strikes.
After returning to the clubhouse, Guillen took to Twitter, first writing, "This one going to cost me a lot money this is patetic (sic)." Moments later, he added, "Today a tough guy show up a yankee stadium."Despite the suspension, Guillen says he will continue to post messages on the social networking service.
"I will continue to Twitter, just not during the game," said Guillen. "I've been tweeting for two years, that's the first time I ever, ever tweeted about baseball. Look at what happened. I learned my lessson. But I'm going to [use] Twitter, that's my private life."
Konerko says he's happy to have a manager that watches his back.
"It's always nice when your manager argues for it," said Konerko. "It was kind of a weird thing. I don't even know what happened between him and the umpire. I said something to the umpire and I was pretty much done with it.
"That stuff is kind of beyond me, as far as Twitter and all that stuff. Ozzie is gonna do what he's gonna do. I think we can all agree at this point in time that no one is going to stop Ozzie from being Ozzie."
When asked how long it took him to realize that he might have made a mistake in taking his grievances to the Internet, Guillen said it didn't take long.
"Two minutes later," said Guillen. "I knew what I would face. I don't blame anybody in baseball being upset. I made a mistake. I assume [responsibility] for it."
The White Sox began a six-game homestand on Friday, hopeful that a return to their home ballpark will help them break a recent slide of 12 losses in 15 games. Guillen said he'd be following his team, though he was unsure before Friday's game if he was allowed to watch from a suite, or if he had to cut out of the ballpark altogether.
"Obviously, I'm going to watch the game," said Guillen. "I'm not going to have a [fake] mustache. They told me not to be around, and I told them that no matter what I do, they are gonna know because everyone will follow me to see what I'm going to do.
"I hope the players play better without me, because with me, they aren't playing too good."
While Guillen plans to continue tweeting, he pledged to tweet smarter.
"I tweet just because it's fun," said Guillen. "I think it's part of the game. I'm not the only manager that tweets. A lot of managers tweet about the team more than me. I never tweet about the team. This was the first time and it cost me a lot of money and a two-day suspension."
Bradford Doolittle is a contributor to MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.